Document Type
Article
Publication Date
1-28-2021
Publication Title
Scientific Reports
Volume
11
Issue
1
First page number:
1
Last page number:
12
Abstract
© 2021, The Author(s). During sporulation Bacillus subtilis Mfd couples transcription to nucleotide excision repair (NER) to eliminate DNA distorting lesions. Here, we report a significant decline in sporulation following Mfd disruption, which was manifested in the absence of external DNA-damage suggesting that spontaneous lesions activate the function of Mfd for an efficient sporogenesis. Accordingly, a dramatic decline in sporulation efficiency took place in a B. subtilis strain lacking Mfd and the repair/prevention guanine oxidized (GO) system (hereafter, the ∆GO system), composed by YtkD, MutM and MutY. Furthermore, the simultaneous absence of Mfd and the GO system, (i) sensitized sporulating cells to H2O2, and (ii) elicited spontaneous and oxygen radical-induced rifampin-resistance (Rifr) mutagenesis. Epifluorescence (EF), confocal and transmission electron (TEM) microscopy analyses, showed a decreased ability of ∆GO ∆mfd strain to sporulate and to develop the typical morphologies of sporulating cells. Remarkably, disruption of sda, sirA and disA partially, restored the sporulation efficiency of the strain deficient for Mfd and the ∆GO system; complete restoration occurred in the RecA− background. Overall, our results unveil a novel Mfd mechanism of transcription-coupled-repair (TCR) elicited by 8-OxoG which converges in the activation of a RecA-dependent checkpoint event that control the onset of sporulation in B. subtilis.
Disciplines
Bacteriology | Biochemistry
File Size
1935 Kb
Language
English
Rights
IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Repository Citation
Suárez, V.,
Martínez, L.,
Leyva-Sánchez, H.,
Valenzuela-García, L.,
Lara-Martínez, R.,
Jiménez-García, L.,
Ramírez-Ramírez, N.,
Obregon-Herrera, A.,
Cuéllar-Cruz, M.,
Robleto, E.,
Pedraza-Reyes, M.
(2021).
Transcriptional Coupling and Repair of 8-OxoG Activate a RecA-Dependent Checkpoint That Controls the Onset of Sporulation in Bacillus subtilis.
Scientific Reports, 11(1),
1-12.
http://dx.doi.org/10.1038/s41598-021-82247-8