Document Type
Article
Publication Date
6-30-2022
Publication Title
Nature Communications
First page number:
1
Last page number:
13
Abstract
Trace metals have been an important ingredient for life throughout Earth’s history. Here, we describe the genome-guided cultivation of a member of the elusive archaeal lineage Caldarchaeales (syn. Aigarchaeota), Wolframiiraptor gerlachensis, and its growth dependence on tungsten. A metagenome-assembled genome (MAG) of W. gerlachensis encodes putative tungsten membrane transport systems, as well as pathways for anaerobic oxidation of sugars probably mediated by tungsten-dependent ferredoxin oxidoreductases that are expressed during growth. Catalyzed reporter deposition-fluorescence in-situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (nanoSIMS) show that W. gerlachensis preferentially assimilates xylose. Phylogenetic analyses of 78 high-quality Wolframiiraptoraceae MAGs from terrestrial and marine hydrothermal systems suggest that tungstenassociated enzymes were present in the last common ancestor of extant Wolframiiraptoraceae. Our observations imply a crucial role for tungsten-dependent metabolism in the origin and evolution of this lineage, and hint at a relic metabolic dependence on this trace metal in early anaerobic thermophiles.
Keywords
Compact radiation sources; Radio transient sources; Radio bursts; Radio continuum emission; Interstellar medium
Disciplines
Life Sciences
File Format
File Size
3500 KB
Language
English
Rights
IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Repository Citation
Buessecker, S.,
Palmer, M.,
Lai, D.,
Dimapilis, J.,
Mayali, X.,
Mosier, D.,
Jiao, J.,
Colman, D. R.,
Keller, L. M.,
John, E. S.,
Miranda, M.,
Gonzalez, C.,
Gonzalez, L.,
Sam, C.,
Villa, C.,
Zhuo, M.,
Bodman, N.,
Robles, F.,
Boyd, E. S.,
Cox, A. D.,
Clair, B. S.,
Hua, Z.,
Li, W.,
Reysenbach, A.,
Stott, M. B.,
Weber, P. K.,
Pett-Ridge, J.,
Dekas, A. E.,
Hedlund, B. P.,
Dodsworth, J. A.
(2022).
An Essential Role for Tungsten in the Ecology and Evolution of a Previously Uncultivated Lineage of Anaerobic, Thermophilic Archaea.
Nature Communications
1-13.
http://dx.doi.org/10.1038/s41467-022-31452-8